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Image Search Results
Journal: Frontiers in Physiology
Article Title: FasL Modulates Expression of Mmp2 in Osteoblasts
doi: 10.3389/fphys.2018.01314
Figure Lengend Snippet: Correlation of FasL expression with markers of bone cells in prenatal (E15) mandibular bone. Distribution of osteocalcin-positive cells/osteoblasts (A,D) , localization of FasL expression (B,E) , localization of TRAP-positive cells/osteoclast (C,F) . Positive cells (immunohistochemistry-brown, TRAP-purple), white arrows point to the osteoclasts, yellow arrows point to the osteoblasts. Scale bar = 50 μm.
Article Snippet: The following primary antibodies were applied:
Techniques: Expressing, Immunohistochemistry
Journal: Frontiers in Physiology
Article Title: FasL Modulates Expression of Mmp2 in Osteoblasts
doi: 10.3389/fphys.2018.01314
Figure Lengend Snippet: Regulation of Mmp2 expression in osteoblastic precursors (MC3T3-E1) using rmFasL/anti-FasL. Immunoflourescent detection of actin filaments (A), FasL (B) , Mmp2 (C) , and Sost (D) in MC3T3-E1 cells. Actin filaments, FasL, and Mmp2 positive cells are green, nuclei are blue. Expression of Mmp2 (mRNA) after: 3 days of rmFasL application compared to untreated cells resulted in 15% increase, p = 0.0295 (E) , 6 days of rmFasL application compared to untreated cells resulted in 30% increase, p = 0.0210 (F) , 3 days of anti-FasL application compared to untreated cells resulted in 14% decrease, p = 0.0024 (G) , 6 days of anti-FasL application compared to untreated cells resulted in 20%, p = 0.0048 (H) , 3 days of anti-FasL application/3 days of rmFasL treatment compared to untreated cells resulted in 27% increase, p = 0.0303 (I) , 3 days of anti-FasL application/3 days of rmFasL treatment compared to 3 days of anti-FasL application/3 days of untreated culture resulted in 23% increase, p = 0.0262 (J) . For each group n = 3. Scale bar = 100 μm. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01.
Article Snippet: The following primary antibodies were applied:
Techniques: Expressing